This article presents a simple and systematic approach to HPLC method development, beginning with sample preparation and finishing with practical analytical method validation. step 5 - method ...

Modern HPLC method development is dominated by a small number of pH adjusting reagents and buffers that are prevalent even when the method uses UV detection. This is driven primarily by the ...

We recently introduced a new series of UHPLC columns with a particle size of 1.6 µm. Here we explain how to transfer an existing HPLC method to a new UHPLC method using polyphenols as an example.

There are a few scenarios where analytical procedures might need to be transferred between HPLC instruments: 1) Method transfer to new equipment in the same lab, perhaps to a different vendor or a ...

High Performance Liquid Chromatography (HPLC) is an analytical procedure for separation, identification and quantification of substances using liquid chromatography (LC). The mixture for separation is ...

Metabolite profiling plays a crucial role in drug discovery and development, and HPLC-Q-TOF has evolved into a powerful and effective high-resolution analytical tool for metabolite detection. However, ...

When conducting reproducible and interference-free analysis by liquid chromatography, the purity of the solvents used, particularly the purity of water, is crucial for the sensitivity of this method, ...

Radio-HPLC is a powerful tool for analyzing radioactive species in radiopharmaceutical chemistry. In this paper, we found an example that the commonly used eluting solvent, acetonitrile, could ...

Investigation of peak purity failure during HPLC method validation led to discovery of a co-eluting impurity under the main peak. Spectral analysis, including three-dimensional modelling, was used to ...